Édesvizi algák által termelt cianotoxinok hatásai vizi gerinctelen szervezetekben – idegrendszeri háttérmechanizmusok in vivo és in vitro modell rendszereken = Neuronal substrate(s) of cyanotoxin effects produced by freshwater algae - in vivo and in vitro studies on model freshwater invertebrate organisms
2011
Termeszetes fitoplankton tomegprodukcio soran gyűjtott mintak es hazai cianobakterium torzsek in vivo toxikologiai elemzese (THAMNOTOX-F, ArTox, DAPHTOX-FTM, ToxAlert 100) ill. in vitro biomarker meresei bizonyitjak, hogy sporadikusan mind a Balatonban mind a Kis-Balaton teruleten megjelenhetnek potencialisan toxikus algatorzsek. A Balatonbol izolalt Cylindrosprmopsis raciborskii torzsek toxikus mechanizmusai a mert biokemiai parameterek (LDH, GST szintek, AchE gatlas) alapjan elternek a cilindrospermopszint termelő ausztral C. raciborskii (AQS) torzs hatasatol, es jobban hasonlitanak az anatoxinokat termelő Oscillatoria (PCC 6505) torzsre. Puhatestű neuronokon kapott elektrofiziologiai eredmenyek (az algakivonatok kolinerg receptorblokkolo, esetenkent AchE gatlo hatasa) is a PCC torzs hatasahoz merhető. In vitro, CHO-K1 sejttenyeszeten vegzett eletkepessegi tesztek es a sejtvaz szerkezeteben kialakulo degenerativ valtozasok rovid-es hosszutavu (feltehetően elterő bioaktiv komponensek vagy mechanizmusok reven) kialakulo citotoxikus hatasokat is kimutattak. Molekularis genetikai vizsgalatok szerint a balatoni C. raciborskii torzsek nem termelnek cilindrospermopszint, es analitikai vizsgalatok sem igazoltak eddig ismert cianotoxinok (anatoxin-a, anatoxin-a(S) vagy szaxitoxinok) jelenletet. Az in vivo es in vitro tesztek szerint okozott toxicitas igy feltehetően egyeb, eddig nem azonositott toxikus metabolitok jelenletenek, illetve ezek szinergista hatasanak koszonhetőek. | Cyanobacterial bloom samples and endogenous cyanobacterial isolates were assessed for environmental risk by a battery of in vivo toxicity tests (THAMNOTOX-F, ArTox, DAPHTOX-FTM, ToxAlert 100) and in vitro biochemical assays of test animals. The results revealed the sporadic appearance of toxic strains of cyanobacteria in the lake Balaton and Kis-Balaton area. Biochemical data (LDH, GST and AchE levels) suggested different toxic mechanisms of the extracts of Cylindrospermopsis raciborskii isolates from the Lake Balaton compared with the cylindrospermopsin producing Australian C. raciborskii strains. The electrophysiological results on identified gastropod neurons showed similar features (cholinegic blocking and AchE inhibiting effects) with the extracts made from the anatoxin-a producing Oscillatoria (PCC 6505) strains. In vitro viability tests and morphological studies on CHO-K1 cell culture separated the short term and long term cytotoxic effects of the C. raciborskii strains, probably due to their different toxic components and target mechanisms. Molecular genetic studies did not prove the presence of cylindrospermopsin, neither analitical analysis showed any of the recently known cyanotoxins (anatoxin-a, anatoxin-a(s), saxitoxin) in the extracts of C. raciborskii strains from Balaton, suggesting that their in vivo and in vitro toxicity is the product of other, recently unidentified toxic substances and their likely synergistic interactions.
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