Telomerase activity in myeloma cells is closely related to cell cycle status, but not to apoptotic signals induced by interferon-α

Telomeres, G-rich structures at the ends of chromosomes are essential for maintaining chromosomal integrity. Most tumor cells contain telomerase, a ribonucleoprotein that elongates telomeric repeats, and it plays an essential role in indefinite proliferation. To better understand regulatory mechanisms of telomerase, in relationship with apoptosis and the cell cycle, we examined telomerase activity in PCM6, an interleukin-6 (IL-6)-responsive, interferoncl (IFN-α)-sensitive multiple myeloma cell line, using a PCR-based assay. When PCM6 cells were cultured in serum-free media, the addition of IFNα resulted in apoptosis of the cells, but with no influence on telomerase activity. When IFNα was added to the culture with serum plus rIL-6 after serum deprivation, G1-S transition was inhibited and telomerase activity was lower compare to findings in culture with no IFN-α. Dose response experiments of rIL-6 and IFN-α, and the measurement of telomerase activity of sorted cells in S-phase using CD71, demonstrated a h...