Serum proteomics profiling for diagnosis of myasthenia gravis

Objective To identify the significant protein peaks and establish the diagnostic model of myasthenia gravis (MG) by serum proteomics profiling analysis. Methods The serum samples from 56 MG patients and 16 healthy controls were detected by the technology of surface-enhanced laser desorption ionization time-of-flight mass spectrometry (SELDI-TOF MS). The differentially expressed protein peaks were identified to establish a MG diagnostic model And preliminary validation was performed. Results Thirty-eight specific protein peaks with significant differences were found in the serum protein pattern of 56 MG patients and 16 healthy controls. Systemic optimization identified 2 protein peaks of M4168.94 and M1122. 57. And they were used to build the MG diagnostic model of differentiating 56 cases from 16 controls. Conclusion The serum protein profiling can be a novel, effective and sensitive tool to screen for MG-related protein peaks and establish a diagnostic model. Key words: SELDI-TOF-MS;  Serum protein profiling;  Myasthenia gratis;  Diagnosis