An apparatus for optical examination of a sample, The method for inspecting a sample and methods for displacing a device in an operational state

The invention relates to an apparatus for optically inspecting a sample with a Kryoimmersionsobjektiv, with a tripod on which the Kryoimmersionsobjektiv is attached, wherein the Kryoimmersionsobjektiv having a plurality of optical components, in particular lenses, and wherein the Kryoimmersionsobjektiv has an optical face component, which in operation communicates with a coolable immersion liquid in contact with a sample support for an assayed sample, comprising means for providing a cooled immersion liquid between the optical front component and of the test sample on or at the sample carrier. The apparatus is characterized in that insulating means are provided for interrupting a heat transfer between the stand and the front optical component. The invention also relates to a method for inspecting a sample, a plurality of microscopic images is taken at the with an inventive device, in each case, a different offset between a main body and a separate housing is adjusted for the individual images. Finally, the invention relates to a method for placing a device according to the invention in an operational state in which the cooled during operation components of the Kryoimmersionsobjektivs, in particular the optical front component are cooled by a coolant, in particular liquid nitrogen, wherein the immersion liquid with a coolant , in particular, cooled with liquid nitrogen, and in which thereafter the cooled components of the Kryoimmersionsobjektivs are brought into contact with the immersion liquid.