Einfluss der Verfahrenstechnik auf die Eliminierung ausgewählter Estrogene und Xenestrogene in Kläranlagen: ein BMBF Verbundprojekt: Untersuchungen zur Elimination von Bisphenol A in Laborkläranlagen und der estrogenen Wirkung gereinigten Abwassers

Experiments with a coupled unit OECD 302 (DIN 38412 L26) biodegradation test system showed that influent concentrations of 1mg/L Bisphenol A (BPA) were reduced to concentrations < 200 ng/l in the effluent after biological treatment. These data confirm an approximately complete elimination of the compound in course of sewage treatment. Estrogenic effects of BPA on zebrafish (Danio rerio) were estimated by a vitellogenin assay. Aprotinin stabilised serum proteins were separated by SDS-PAGE. An antiserum to highly purified vitellogenin of carp (Cyprinus carpio) has been used for immunological identification of the vitellogenin in Western blot. Flow through exposure of male zebrafish to the effluent of 1 mg/I BPA spiked coupled unit test systems during the steady state phase of biodegradation for a 21 d period caused a weak induction of vitellogenesis. In order to check whether the estrogenic effects of the effluent are related to BPA degradation products or to the low concentrations of the parent compound, exposure tests with BPA using charcoal filtered tab water were carried out. Semi static exposure to 500 ng/l BPA (nominal concentrations) for 14 days also induced vitellogenesis in male zebrafish. Due to the fact, that effective concentrations of BPA are in the same range as BPA concentrations measured in German wastewater treatment plant effluents, biodegradability of low concentrations of BPA in surface waters as well as estrogenic capacity of the compound should be investigated more detailed.