Waveguide fluorosensor for the detection of ligand-receptor interactions - The 5-HT3 serotonin receptor
1999
A highly-sensitive surface fluorescence technique was combined with a powerful method for the oriented reversible immobilization of membrane proteins on oxide surfaces. Affinity and kinetic parameters of the interaction of the receptor and a fluorescent ligand were evaluated. The approach is particularly suited for the study of rapid ligand-receptor interactions where optimized cell volume, flow and injection conditions are required.
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