New Enhanced Method for Determination of Trace Sulfamethodxazole Based on the Fluorescence Behaviors of Cyclodextrins in Water Solutions.

The widespread occurrence of sulfonamides (SAs) in the environment water has rasied great concerns about their potential to antibiotics resistance. In this study, the fluorescence behaviors of sulfamethoxazole (SMZ) representing certain properties of the SAs mixed with three different kinds of cyclodextrins (CDs) in water solutions were investigated, respectively. The result reported that the shapes of the fluorescence peak and its position for the SMZ that were mixed with the CDs were almost the same as those of the standard SMZ, respectively. In addition, compared with the identical control sample the fluorescence of SMZ mixed with each of the CD was greatly enhanced. Therefore, a new simple, and sensitive spectrofluorimetric method for the determination of SMZ was established in water solutions. and the dynamic linear ranges varied from 0.01 to 0.7 mg/L with the detection limit of 7.1 ng/L. And the correlation coefficient was more than 99.9%. Significantly, this new method was successfully applied to direct determination of SMZ in pharmaceutical compounds. Moreover, the results showed that the SMZ could separately form the 1:1 supramolecular compound with each of the CD.