Effect of metabolic inhibitors on K+ transport across the lamprey (Lampetra fluviatilis) erythrocyte membrane.

In order to assess the contribution of oxidative metabolism to K + ( 86 Rb +) transport across the lamprey red cell membrane, the effects of vari­ ous metabolic inhibitors were examined. The influx of K + was reduced markedly in the presence of 20 /imol/1 2,4-dinitrophenol (2,4-DNP) or rotenone, and to a lesser extent by 1 mmol/1 cyanide. Rotenone produced complete inhibition of the K+ active transport and a partial blockade of K + channels by 28% on the aver­ age. Addition of 2,4-DNP to incubation media resulted in a significant reduction of both active transport of K + (by 47 %) and of K + movement via channels (by 57%). The inhibitory effect of 2,4-DNP on total K+ influx was independent on decreasing extracellular pHe from 7.4 to 6.5. The blocking action of 1 mmol/1 Ba 2+ on K + channels was abolished in the red cells incubated at pH e 6.5. Treatment of the red cells with 1 mmol/1 cyanide diminished active transport of K + to about 34% of control values but did not affect K+ channels. The obtained data indicate that in the lamprey red blood cells at least a half of energy needed for the active transport of K + is supplied with ATP produced by oxidative phosphorylation. It may be suggested that NADH dehydrogenase is the key enzyme required for active transport of K + in the cells, as rotenone, a selective blocker of this enzyme, causes a complete blockade of the Na +, K + -pump.