Method for manufacturing heterodimeric antibody molecules Fc using electrostatic effects driving

Heterodimeric protein comprising a first polypeptide containing CH3 IgG and a second polypeptide containing CH3 IgG which bind to form an interface designed to promote the formation of heterodimers, in which said first polypeptide containing CH3 IgG it comprises a substitution of amino acid at position 392 with an amino acid negatively charged, and said second polypeptide containing IgG CH3 comprises a substitution of Asp399, Glu356, Asp356, or Glu 357 with an amino acid of positive charge.