Confirmation of SSR Markers and QTL for Seed Calcium Content and Hardness of Soybean

ABSTRACTSeed calcium content and hardness constitute determining characteristics of soybean [Glycine max (L.) Merr.] texture affecting soyfood quality. Molecular markers linked to these traits will accelerate breeding of soybeans for the soyfood market through the use of marker-assisted selection (MAS). Confirmation of linked markers and validation of quantitative trait loci (QTL), prior to their application through MAS, are the keys. The objectives of this study were to confirm previously reported QTL for calcium content and seed hardness and investigate the relationship between these traits. Evaluation of seven recombinant inbred line (RIL) populations with different genetic backgrounds, grown in two Arkansas locations for 2 years, showed inconsistent correlations between these traits. In general, a positive correlation was found in most of the populations and correlation was significant in six populations. Combined data showed a positive correlation between calcium content and seed hardness (r = 0.23 –...