IN VITRO REGENERATION OF MONOEMRBYONIC MANGO CULTIVARS

Different explants of mango cultivars were explored for in vitro plantlet regeneration on Murashige and Skoog (MS) and Gamborg (B5) medium. Only zygotic embryos of Chaunsa cultivar initiated callus formation and successful plant regeneration with highest percentage on MS medium supplemented with 2,4-D 1mgL-1. Calli were also observed from zygotic embryos of cv. Anwar Rataul but could not regenerate into plantlets. Nucellus and shoot tip explants of both cultivars showed no callus induction because of excessive phenolic exudation from explant in the medium. The plantlets generated through embryo culture were transplanted to green house after acclimatization. Further studies are suggested, however, to optimize somatic embryogenesis from nucellar explants of mango for clonal propagation and biotechnology applications.