Targeted base editing in rice with CRISPR/ScCas9 system

: The CRISPR/Cas system has rapidly become the preferred tool for genome engineering in various organisms due to high efficiency, specificity, simplicity and versatility. Currently, CRISPR/Cas-mediated base editing, a novel genome editing strategy that enables irreversible nucleotide changes at target loci without double-stranded DNA cleavage or any donor template, has been widely adopted for generating gain-of-function germplasms in functional genomics research and crop genetic improvement (Hua et al., 2019; Ren et al., 2018; Yan et al., 2018).