Construction of Prokaryotic Expression Vectors Harboring HV S and S Partial Gene Segments and Identification of Expressed Nucleocapsid Protein

Abstract：To study the antigenicity of prokaryotic expressed products of Hantavirus(HV)S partial gene(502—1326bp)an d obtain data for serotyping antigen，the prokaryotic expression vectors pRSET A—S，pRSET A—S—C were constructed respectively．The recombinant expression plasmids were fans— formed into BL2 I(DE3)pLySs．Th e expression product were analyzed by SDS—PAGE and Western—blot． Th e results showed that the proteins were expressed at high level respectively an d could be recognized by patients’(infected by HTNV)sera distinctly and peculiarly．Th e truncated NP(aa155 to 429)could be considered as serotyping an tigen．