Cloning and phylogenetic analysis of interferon-α gene of Jingba canine.

One pair of primers was designed and synthesized according to the canine interferon alpha gene(IFN-α) nucleic acid sequence(AB102731) published in the GenBank.Jingba canine IFN-α gene was amplified by PCR,then cloned and sequenced.The results showed:cloned canine IFN-α gene span was 564 bp,which included one open-reading frame,encoding 187 amino acid residues,three potential N-glycosalation sites and ten cysteines in the deduced amino acid sequence.The results of homology analysis with other canine interferon-alpha gene published previously in the GenBank by DNAStar analysis revealed that there was identity of 96.3% at nucleotide homology and that of 94.1% at amino acid homology.The results of comparative sequence analysis and phylogenetic tree analysis indicated:there was difference of genus in IFN-α gene,the nearer relationship,the higher homology.This study paved the way for future study of biological function and application of canine IFN-α.