Cytomorphologic results of preparation experiments for monolayer deposition of cervical material.

For automated presscreening methods by high resolution analysis serving as a detecting method in gynecologic mass screening programs a new monolayer deposition method of cervical material has been used. This method will be outlined briefly and the mode of evaluation as well as current cytomorphological findings will be presented. With regard to measurability of the slides prepared according to the new method a number ofcytologic criteria were thought to be ofparticular importance. These criteria are delineated and compiled in a table. A form in which these criteria were listed was filled in by cytopathologists for each slide evaluated. When performing isolation and centrifugation procedures several new morphologic questions arose to the cytopathologist which can only partly be answered by now. If taking into account all criteria of evaluation it may be followed from the present experiences that slides of cervical material are much more suited for automated prescreening methods by high resolution analysis if prepared after isolation and centrifugation in macromolecular liquids than are conventional Papanicolaou smears or slides from suspensions with isolated cells that were not subjected to centrifugation procedures. When using high resolution analysis as an automated prescreening method in gynecologic mass screening programs, a number of cytologic demands have to be fulfilled as have to in other automated prescreening programs (2, 5, 7). 1. The isolation of representative cervical material which in conventional smears mostly is clustered.