Label-free Electrostatic Detection of DNA Amplification by PCR Using Capacitive Field-effect Devices
2016
Abstract A capacitive field-effect EIS (electrolyte-insulator-semiconductor) sensor modified with a positively charged weak polyelectrolyte of poly(allylamine hydrochloride) (PAH)/single-stranded probe DNA (ssDNA) bilayer has been used for a label-free electrostatic detection of pathogen-specific DNA amplification via polymerase chain reaction (PCR). The sensor is able to distinguish between positive and negative PCR solutions, to detect the existence of target DNA amplicons in PCR samples and thus, can be used as tool for a quick verification of DNA amplification and the successful PCR process.
Keywords:
- Polymerase chain reaction optimization
- Multiplex polymerase chain reaction
- Multiple displacement amplification
- Primer dimer
- Ligase chain reaction
- Recombinase Polymerase Amplification
- Engineering
- Analytical chemistry
- Real-time polymerase chain reaction
- Polymerase chain reaction
- Molecular biology
- Amplicon
- Composite material
- Chromatography
- Correction
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