[Transcriptional expression of apoptosis suppression gene bcl-2 in non-small cell lung carcinoma].

Objective To investigate the role of Bcl 2 gene in oncogenesis and progression of non small cell lung carcinoma(NSCLC). Methods The mRNA expressions of Bcl 2 gene in a series of 137 pulmonary tissues collected at various sites and with different properties were studied with Northern hybridization and non radioactive digoxigenin labeling and detection system. Results According to the observations on benign lesions, non cancer tissues distant from tumour, para tumour tissues and cancer tissues, there was a tendency of mRNA expression increasing of Bcl 2 gene. Among them, Bcl 2 mRNA expression in lung cancer tissues was significantly increased, compared with that in benign lesions and tissues distant from tumour ( P 0.01). The Bcl 2 mRNA expression in lymph node with cancer metastasis was significantly higher than that in lymph node without cancer metastasis( P 0.05). The Bcl 2 mRNA expression was relative to cell differentiation of the cancer, but not to the histological classification.Conclusion Over expression of Bcl 2 gene might be involved in the oncogenesis and metastasis of NSCLC.