Prevalence of Scedosporium species and Lomentospora prolificans in patients with cystic fibrosis in a multicenter trial by use of a selective medium

Abstract Objective Detection of hyphomycetes of the Scedosporium apiospermum complex and Lomentospora prolificans ( Sa c- Lp ) is not yet standardized. Prevalence rates in patients with cystic fibrosis (CF) and the resistance pattern of these pathogens in Germany are unknown. Methods In a one-year prospective study 11 laboratories used a selective medium for isolation of Sa c- Lp , examining >11,600 respiratory samples from 2346 patients with CF. Isolates were identified by molecular methods and tested for susceptibility to antifungal drugs. Results The prevalence of Sa c- Lp in patients with CF in Germany varied from 0.0 to 10.5% (mean: 3.1%) among the clinical centres. The benefit of the selective medium SceSel + compared to standard media for fungi was documented for >5000 samples. High antifungal resistance was detected in the S. apiospermum complex, and the multiresistance of L. prolificans was confirmed. Conclusion Microbiology laboratories should be aware of these resistant species in patients with CF and consider using a selective medium.