Molecular epidemiology of Mycobacterium tuberculosis isolates with spoligotyping method

Abstract Background Spoligotyping can assay the transmission of Mycobacterium tuberculosis strains. We aimed to study the geneotyping of M. tuberculosis isolated from patients with tuberculosis from west of Iran by spoligotyping. Methods A total of 47 M. tuberculosis isolates were collected from west of Iran. All sample cultures on Lowenstein-Jensen medium and incubated at 37 °C for 8 weeks. Bacterial isolates were identified as M. tuberculosis using standard biochemical tests. Drug resistance patterns of M. tuberculosis were to rifampicin and isoniazid determined and multi drug resistance (MDR) strains isolated. After DNA extraction, spoligotyping was performed. Results This study revealed new spoligotypes 4162 and 4163 that correlated to atypic lineage. Atypic and unknown lineages also had correlation with MDR-TB rate (4%). The most prevalent (Spoligotyping international types) SIT were Orphan (34%), 2669(23.4%) and 127(14.8%) types. The most prevalent clade were Ural-2 (NEW-1) (25.53%) and atypic (23.40%) lineages. Conclusion The predominant clade was Ural-2 (NEW-1) and Atypic lineages lineage that restricted to Iran. Also the rate of MDR were so low. A good control program need to know the dynamic transmission of local isolates, therefore this study presents the circulating isolate in west of Iran.