The effect of mechanical vibration on orthodontically induced root resorption

ABSTRACT Objective:  To investigate the effect of low-frequency mechanical vibration (LFMV) on orthodontically induced root resorption. Materials and Methods:  Forty male CD1, 12-week-old mice were used for the study. The mice were randomly divided into five groups: group 1 (baseline)—no spring and no mechanical vibration, group 2—orthodontic spring but no vibration, group 3—orthodontic spring and 5 Hz of vibration applied to the maxillary first molar, group 4—orthodontic spring and 10 Hz of vibration applied to maxillary first molar, and group 5—orthodontic spring and 20 Hz of vibration applied to maxillary first molar. In the different experimental groups, the first molar was moved mesially for 2 weeks using a nickel-titanium coil spring delivering 10 g of force. LFMVs were applied at 5 Hz, 10 Hz, and 20 Hz. Microfocus X-ray computed tomography imaging was used to analyze root resorption. Additionally, to understand the mechanism, we applied LFMV to MC3T3 cells, and gene expression analyses were done fo...