Study on Extraction and Antioxidation Property of Polyphenol from Chestnut Shells

The effects of different factors on extraction of polyphenol from chestnut shell were studied,and the antioxidation pro-perty of polyphenol of chestnut shell was also studied.The results showed that the optimum extraction conditions were:ethanol volume fraction 59.7%,solid-liquid ratio 1∶18(g∶mL),extraction temperature 52.6℃ and extract 3 times.The predicted yield of chestnut shell polyphenol is 76.0 mg/g and the test result is 75.9mg/g.Polyphenol in chestnut shell has strong ability to eliminate free radicals.The abilities of reducing and removing DPPH·,·OH,O-2· gradually increased with the increase of concentration in the range of 25 to 200mg/L.The ability of reduction reached 0.841,and the rate of inhibition reached 89.7% at 200mg/L,which are higher than those of 2,6-di-tert-butyl-p-cresol(BHT) but lower than those of VC,at similar mass concentration.The removal rates of O-2· and ·OH are 93.2% and 94.0%,respectively,at concentration of 200mg/L,which are higher than BHT and Vc.