Method for preparing teriparatide by fragment method and solid-liquid combination

The invention belongs to the field of polypeptide synthesis, relating to a method for preparing teriparatide by solid-liquid combination. The method adopts a liquid phase mode to synthesize a part of dipeptide and tripeptide fragments, feeding is performed by the synthesized dipeptide and tripetide fragments, 13 steps of solid phase coupled reaction are reduced, the coupling efficiency is greatly improved, not only is the generation of racemization impurities difficult to be purified and removed at the 34th site tail end avoided, but also the generation of missing peptides at multiple sites is avoided, and the purity of the final target peptide reaches 75 percent or more. Compared with the prior art, the method provided by the invention is simple in synthetic route, less in solid phase coupling steps, mainly solves the problems that a peptide sequence is too long and coupling is difficult, and that the missing peptides are easily generated, the fragment synthesis technology is well developed, the materiel cost is lowered, and the industrialized mass production can be carried out.