AMN082, an allosteric mGluR7 agonist that inhibits afferent glutamatergic transmission in rat basolateral amygdala.

Abstract Glutamatergic neurotransmission has been implicated in the pathophysiology of psychiatric disorders, such as anxiety and depression. The possible contribution of group III metabotropic glutamate receptors has been poorly investigated, due to the lack of selective pharmacological tools. However, a selective agonist of mGLUR 7 , AMN082 has been identified recently, and shown to act through an allosteric mechanism in recombinant cells expressing the receptor. Thus, using AMN082, we examined the role of mGLUR 7 in modulating synaptic transmission in the rat basolateral amygdala (BLA), a brain region known to be important for the genesis of anxious states. We found that bath application of AMN082 (1–10 μM) produced a concentration-dependent inhibition of synaptic transmission evoked at 2 Hz, but had no effect on transmission evoked at 0.05 Hz. However, at this lower frequency, AMN082 (10 μM) significantly increased the synaptic inhibition produced by a group III mGLUR agonist, L-AP4 (100 μM). This effect was blocked by pre-application of CPPG (500 μM), a group III mGLUR-preferring antagonist, consistent with the involvement of mGLUR 7 . Thus, we have shown that AMN082 can modulate high frequency synaptic transmission in the BLA, in vitro, and appears to act on the receptor via an allosteric mechanism. These results suggest that mGLUR 7 has a unique role in regulating neuronal activity in the BLA and may be a target for novel drugs for the treatment of anxiety.