In vitro activity of matrine against Candida albicans biofilms

PURPOSE:To establish a model of Candida albicans biofilms and to examine the effect of matrine on C.albicans biofilms and ultrastructure.METHODS:C.albicans collection strain ATCC76615 was obtained and propagated.Biofilms were formed in 96-well microtiter plates.Antifungal susceptibility testing of C.albicans biofilms were assessed with the tetrazolium salt(XTT)reduction assay.Confocal laser scanning microscopy(CLSM) and dead/live fluorescent staining technique were combined to detect the effects of Matrine on preformed C.albican biofilms’ composition and ultrastructure.RESULTS:Matrine was active against different growth stages(early、middle、mature)of biofilms;The bioactivity and drug-resistance of C.albican biofilm increased with culturing time.CLSM showed that C.albicans biofilms were inhibited and growth were predominantly composed of yeast cells and pseudohyphae.CONCLUSIONS:This study demonstrates that Matrine has potent activity against C.albicans biofilms in vitro and potential therapeutic implication for biofilm-associated candidal infections.