Subtyping of male breast cancer by PAM50 and immunohistochemistry; a pilot study of a consecutive Danish cohort.

2020 
Male breast cancer (MBC) is a rare disease that is still to be fully understood. In female breast cancer molecular subtyping by gene expression has proven its significance. In this study we characterize a consecutive cohort of MBC patients surgically treated from 1997-2017, identified at our institution (N=37), and report the association between molecular subtypes found by a surrogate panel of immunohistochemical (IHC) markers, and the PAM50 signature, as well as Risk-Of-Recurrence score and overall survival for the different subtypes. PAM50 subtypes were determined using the nCounter Flex system instrument and software. The distribution of molecular subtypes according to the PAM50 signature was: 56% luminal B, 39% luminal A and 5% basal-like. None of the tumors were HER2-enriched. Using IHC surrogate markers we found 80% luminal B-like, 15% luminal A-like and 5% basal-like. None were HER2 positive (non-luminal). We found a strong statistical association between subtypes found by PAM50 signature and the IHC surrogate markers (P < 0.001). Furthermore we found luminal A tumors to be smaller in size compared to luminal B tumors (P = 0.04). Patients with luminal A subtype tumors had the lowest ROR scores with a mean of 39, whereas patients with luminal B subtype tumors had a mean ROR score of 69. Significant worse overall survival for luminal B tumors compared to luminal A tumors was seen (P = 0.02). Male breast cancer seems to be a mainly luminal disease, with luminal B being the most frequent subtype. Further studies are needed to ensure correct therapeutic strategies for this select group of patients.
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