Chlorophyll a/b-Proteins in Their Relation to the Light-Harvesting Complex

In 1966 two research groups (Ogawa et al. 1966; Thornber et al. 1966) were the first to demonstrate that sodium do-decyl sulfate dissociated thylakoid membranes can be separated into three different zones two of which represent chlorophyll-proteins. Analysis of the two chlorophyll-protein zones, which were termed components or complexes I and II, respectively, revealed most, if not all, of the Chl b associated with complex II whilst complex I contained mainly Chl a.