Влияние делеций в промоторе на экспрессию гена глутамилэндопептидазы Bacillus intermedius

Spo0A, DegU, CcpA, приводят к потере чувствительности экспрессии гена gseBi в отношение глюкозо-катаболитной репрессии.The expression of the Bacillus intermedius glutamyl endopeptidase gene was investigated for different length of its regulatory region. It was proved that the change of the length of the promoter region results in the decrease of the gseBi gene expression level and the shift of the protease accumulation maximums in the medium relative to control. It was also found that the enzyme biosynthesis in salt stress conditions does not depend on the length of the 5'-region. However, with the full-length promoter region the strain's productivity is 1.5 times higher. It was ascertained that the reduction of the promoter region up to 365 bp, when there is no potential binding sites for the regulatory Spo0A, DegU, and CcpA proteins, leads to the loss of sensitivity of the gseBi gene expression to the glucose catabolite repression.