CORELATION BETWEEN DURATION TIMES OF CRYOPROTECTANT TOWARD MICE EMBRYO DEVELOPMENT

1,2-Propanediol is considerably less toxic than ethylene glycol, moreover it is used as a cryoprotectant. Purpose of the research was find correlation between duration timcs ol' c ryoprotectanl toward mice cmbryos development. Zygote embryos were vitrified rhrough plunging into the vitriflcation medium which it contained mixture propanediol 30% as cryoprotectant and phosphate buffer saline. Duration ofvitrification times werc 5, 10, and l5 minutes, respectively. Viabilitl oi post drau, errbr)os was assessrlent through inverted microscope everyday till die or damage. Analysis correlation betrveen duration time ofthree groups toward embryos development were compared of both descriptive statistics and test olwithin subiect contrast thlough cslilrated nrarginal nreans and estimated relative marginalmeans. Research shown correlation betweer du:ation times of cryoprotectant and mice embryos development has different form, it depend on facing the data. Linear correlation was assessed on the descriptive data. On the contEry, shown more quadratic than linear on the within subjects data. However, both duration times of cryoprotectant and mice embryo develoDment has correlation.