Effects of duguelin on epithelial-mesenchymal transition and invasion of rectal cancer lovo cells

Objective To investigate the effects of deguelin on epithelial-mesenchymal transition and invasion of rectal cancer lovo cells.Methods Western blotting and real-time quantitative polymerase chain reaction (Real-time PCR) were used to examine the change of E-cadherin,Twist,Snail and Vimen-tin at protein and mRNA levels.Invasive ability was assessed through wound scrach assay and Transwell assay.Results After treated with duguelin 1 μmol/L for 0,24 and 48 h in lovo cells,the relative density ratio of Twist were (1.40 ± 0.07),(0.41 ± 0.07) and (0.30 ± 0.03) respectively ; of Snail were (1.04 ±0.11),(0.16 ± 0.04) and (0.23 ± 0.02) respectively ; of Vimentin were (4.31 ± 0.30),(1.3 ± 0.27)and (1.21 ± 0.21) respectively ; of E-cadherin were (2.62 ± 0.33),(3.96 ± 0.27) and (6.89 ± 0.16)respectively in protein level.In mRNA level,after treated with dugnelin 1 μmol/L for 24 and 48 h in lovo cells,the relative expression of Twist were (0.63 ± 0.09) and (0.50 ± 0.06) respectively ; of Snail were (0.46 ± 0.06) and (0.51 ± 0.19) respectively; of Vimentin were (0.76 ± 0.05) and (0.57 ± 0.04) re-spectively ; of E-cadherin were (1.88 ± 0.27) and (2.29 ± 0.60) respectively.Scrach assay showed that the relative migration rate of Lovo cells treated with duguelin 1μmol/L for 24、48hours were(20.23 ±5.16)％ and(27.94 ±6.66)％ respectively,compared with(45.65 ±4.41)％ and(66.14 ±3.31)％ in control group(P＜0.01).Comparing to (459.0 ± 14 19) cells invaded in control gToup,deguelin decreased invaded cells to (130.3 ± 16.19,P ＜ 0.01).Conclusion The epithelial-mesenchymal transition and invasion of lovo cells can be inhibited strongly by duguelin. Key words: Deguelin;  Rectal cancer;  Epithelial-mesenchymal transition;  Invision