Preventive effect ofcalcium channel blocker in tacrolimus induced nephrotoxicity in rats

：Objective To study the calcium metabolism intacrolimus(FK506)induced rats nephrotoxicity and the preventive effect of calcium channelblocker.Methods Twenty-four Spragueinduced or FK506-induced nephropathy model.Bloodcreatinine,blood electrolytes,renal tissue histopathology(HE stain)and the change ofultrastructural organization in renal cells by transmission electron microscope wereobserved.Results The blood creatinine levels of both CsA and FK506 groups [(36.00±2.61)and(34.17±4.54)μmol/L]were significantly higher than those of the FK506+Dilgroup and control group(all P＜0.05).Theblood calcium levels of both CsA and FK506 groups (2.00±0.04 and 2.05±0.04 mmol/L) weresignificantly lower than those of the FK506+Dil group and control group(all P＜0.05).Theblood creatinine and calcium levels of FK506+Dil group were not significantly differentwith those of control group(P＞0.05).Histopathologyexamination showed cloudy swelling and vacuolization of the renal tubular epithelial cellsand intra-cellular mitochondria swelling and vacuolization in the CsA and FK506groups.However,the pathological changes of the FK506+Dil group were remarkably milder incomparison with the CsA and FK506 groups.Concluum channel blocker,Dil,could prevent theFK506-induced nephrotoxicity. Key words: Tacrolimus; Nephrotoxicity; Calcium channel blocker; Diltiazem