Molecular identification and mapping of a novel stripe rust resistance gene in wheat resistance line CH5389

2018 
portuguesA ferrugem linear causada por Puccinia striiformis e uma das doencas mais destrutivas do trigo no mundo. A linhagem CH5389 e derivada do cruzamento de trigo com Thinopyrum intermedium e confere resistencia a ferrugem linear. Analises geneticas de individuos da populacao F2 e familia F2:3 obtida a partir do cruzamento entre CH5389 e trigo comum suscetivel revelaram que a resistencia a ferrugem linear na linhagem CH5389 foi controlada por um unico gene dominante, designado YrCH5389. Oito marcadores polimorficos SSR e EST-PCR no cromossomo 3AL foram identificados na populacao F2 de CH5389/Taichung29. O gene YrCH5389 foi delimitado pelos marcadores EST BE405348 e SSR Xwmc388 no cromossomo 3AL com distâncias geneticas de 2,2 e 4,6 cM, respectivamente. Analises genomicas comparativas demonstraram que regioes genomicas ortologas do gene YrCH5389 compreendem 990 kb em arroz, 640 kb em braquipodio e 890 kb em sorgo. Com base nas localizacoes dos marcadores, o gene de resistencia foi localizado no cromossomo 3AL-0.85-1.00. Como nao ha genes oficialmente nomeados de resistencia a ferrugem linear no cromossomo 3AL, o YrCH5389 deve ser designado como um gene novo de resistencia. Esses marcadores de ligacao podem ser uteis para a selecao assistida de genotipos de trigo resistentes a ferrugem linear. Palavras-chave: ferrugem linear; analise genetica; gene de resistencia; analise genomicas comparativas; selecao assistida por marcado EnglishStripe rust, caused by Puccinia striiformis is one of the most destructive diseases of wheat worldwide. CH5389 is a wheat-Thinopyrum intermedium derived line conferring stripe rust resistance. Genetic analyses of seedlings of F2 populations and F2:3 families developed by crossing CH5389 and susceptible common wheat revealed that stripe rust resistance in CH5389 was controlled by a single dominant gene that was designated YrCH5389. Eight SSR and EST-PCR polymorphic markers on chromosome 3AL were identified in F2 population of CH5389/Taichung29. The YrCH5389 was flanked by EST marker BE405348 and SSR marker Xwmc388 on chromosome 3AL with genetic distances of 2.2 and 4.6 cM, respectively. Comparative genomic analysis demonstrated that the orthologous genomic region of YrCH5389 covered 990 kb in rice, 640 kb in Brachypodium, and 890 kb in sorghum. Based on the locations of the markers, the resistance gene was located to chromosome deletion bin 3AL-0.85-1.00. Because there are no officially named stripe rust resistance genes on the 3AL chromosome, the YrCH5389 should be designated as a new resistance gene. These linkage markers could be useful for marker-assisted selection in wheat resistance breeding. Key words: stripe rust; genetic analysis; resistance gene; comparative genomic analysis; marker assisted selection
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