Rapid Detection and Phylogenetic Analysis of West Nile Virus as Zoonosis New Emerging Disease in Patients with Fever of Unknown Origin (FUO)in Surabaya

This study aims to perform a rapid detection and phylogenetic analysis of WNV in the serum of patients with clinical isolates with Fever of Unknown Origin. Serum samples in Surabaya came from 2 patients who had been examined by a Specialist Doctors In the June-July, 2013, with symptoms of fever, headache, sore throat, back pain, myalgia, arthralgia, fatigue, conjunctivitis, pain, anorexia, nausea, abdominal pain, diarrhea, and respiratory symptoms. Running RNA extraction procedure and nested PCR. PCR is run in accordance with protocols west nile virus detection kit from Intron. Similarly, primers used are designed specifically for the west nile virus. Interpretation of PCR products using 1% agarose gel (306 bp), and then performed a homology analysis using Genetix Mac Ver. 8.0 with reference WNV isolates from the gene bank (JF920741.1.gnu). This research demonstrated the effective PCR methods for the rapid detection of WNV and diagnosis of WNV infection from clinical isolates represent a breakthrough in the clinical world to many cases of infectious disease that has not been diagnosed (Fever Unknown Origin = FUO). Based on homology analysis, This sample showed 95% with WNV were reported in the gene bank (JF920741.1.gnu). Keywords: PCR, West Nile Virus, FUO, Patients, Mosquitos