In vitro biosynthesis of bacterial peptidoglycan using D-Cys-containing precursors : fluorescent detection of transglycosylation and transpeptidation

Peptidoglycan precursors containing D-Cys at position 4 were polymerised to form a synthetic peptidoglycan layer, which could be fluorescently labelled, providing a new method to monitor peptidoglycan transglycosylation and transpeptidation.