INHIBITORY ACTIVITIES OF CAMELLIA MISTLETOE (K. JAPONICA) EXTRACTS ON PANCREATIC LIPASE, TYROSINASE AND CANCER CELL PROLIFERATION

Objective: The objective of this study was undertaken to examine pancreatic lipase and tyrosinase inhibitory activities of Camellia mistletoe ( Korthalsella japonica ) extracts and conduct their antiproliferative activity on several human cancer (Hela, A375, HCT 116, HepG2 and A549) and normal cells (TK6) lines. Methods: The lipase inhibitory activity of acts of Camellia mistletoe extracts were determined from the hydrolytic reaction of p-nitrophenyl butyrate with pancreatic lipase and cytotoxicity against human cancer and normal cell lines were also evaluated using the MTT assay. The in vitro tyrosinase inhibitor potency of Camellia mistletoe extracts was estimated using mushroom tyrosinase. Results: Both methanol and ethanol extracts of Camellia mistletoe exhibited an inhibitory effect on lipase inhibition activity with IC 50 values of 0.39 and 0.49 mg/ml, respectively. Moreover, Camellia mistletoe extracts were judged to be strongly active against the cancer cell lines, while they had no antiproliferative activity against the normal human cell lines. However, they had little inhibitory effects on tyrosinase activity. Conclusion: Camellia mistletoe extracts could be the good sources of natural lipase inhibition and anticancer agents with a great potential to be used in food and therapeutic fields.