LOW DOSES OF THIOUREA AND THIOMERSAL INDUCES HORMETIC CELL PROLIFERATION CYTOTOXICITY ASSAY

2014 
A study was conducted to observe the cytotoxicity of thiourea and thiomersal on mouse splenocytes by 3-(4,5-dimethylthiazol2-Yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Thiourea and thiomersal were two fold serially diluted to obtain concentrations (ppm) of 1.6421, 0.8211, 0.4105, 0.2053, 0.1026, 0.0513, 0.0257, 0.0128, 0.0064, 0.0032, 0.0 for thiourea and 25.0, 12.5, 6.25, 3.125, 1.5625, 0.78125, 0.39063, 0.19532, 0.09766, 0.04883, 0.0 for thiomersal in a microtitre plate. One hundred microlitre of splenocytes (1×10 7 cells/ml) were added to each concentration of thiomersal and thiourea followed by incubation at 37°C for 16 h in a CO 2 incubator. Later, the viability of the cells was quantified using MTT. The results of cell viability (%) showed that IC50 of thiourea and thiomersal was 0.08681 ppm and 0.069046 ppm, respectively. In both the compounds, hormetic cell proliferation was observed at lower doses. In conclusion, hormetic cell proliferation of splenocytes both by thiourea and thiomersal may account for erratic immune response due to splenocyte proliferation.
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