Covalently Immobilization of Methylomonas Z201 Cells on Glutaraldehyde Activated DEAE Cellulose

Methylomonas Z201 cells have been immobilized on the glutaraldehyde activated DEAE cellulose for the propene epoxidation. The optimum cell to carrier ratio was determined to be 32 mg dry weight cells/g DEAE cellulose. The optimum pH and the optimum temperature of covalently immolilized cells were 7.8 and 40℃ respectively. The MMO activities of free and covalently immobilized cells were increased by 8% and 35% respectivily, when the air was replaced by pure oxygen as the oxygen source. The activation effect of sodium formate on the MMO activity of covalently immobilized cells was less than that of free cells due to the presence of the carrier. The deactivation kinetics indicated that covalently immobilization enhanced the stability of the cells.