Ethanol and acetaldehyde differentially alter extracellular dopamine and serotonin in Aldh2-knockout mouse dorsal striatum: A reverse microdialysis study.

Abstract Dopamine (DA) and serotonin (5-HT) seem to be involved in several of the effects of ethanol (EtOH). Acetaldehyde (AcH), especially in the brain, induces effects that mimic those of EtOH. The purpose of this study was to investigate the effects of local perfusion of EtOH and AcH on extracellular DA and 5-HT in the dorsal striatum of Aldh2-knockout (Aldh2-KO) and wild-type (WT) mice. Aldh2-KO mice were used as a model of aldehyde dehydrogenase 2 deficiency in humans to examine the effects of AcH. Mice were perfused with Ringer’s solution (control), EtOH (100, 200, or 500 mM) and AcH (100, 200, or 500 μM) into the dorsal striatum. Dialysate samples were collected every 5 min, and then analyzed with HPLC coupled to an ECD. We found that local perfusion with 500 mM EtOH increased extracellular levels of DA ( p p