Method for extracting skunk bush polysaccharide by zymohydrolysis
2008
The invention provides a method for extracting cornel polysaccharide through enzymolysis. The method comprises the following steps that cornel dry flesh is crushed to make cornel dry powder; distilled water is added with the mass being 15 to 60 times than that of the cornel dry powder, and an enzyme are added so as to carry out enzymolysis at a temperature of between 35 and 60 DEG C and a pH value of between 3.5 and 7.0; after the enzymolysis is finished, enzyme inactivation is carried out; an extraction solution is separated and purified so as to obtain a cornel polysaccharide extract; the enzyme is a mixture comprising one sort or more than two sorts of (1) cellulase, (2) papain, (3) pectinase and (4) amylolytic enzyme; and the dose by mass of the enzymes accounts for 0.1 to 24 percent of the mass of the cornel dry powder. The method has the advantages that compared with the prior hot water extraction method, the method can increase the extraction rate of cornel polysaccharide by 10 to 50 percent and the content of cornel polysaccharide by 15 to 80 percent; and due to preventing the influence of the prior hot water extraction, acid extraction or alkaline extraction on the structure of polysaccharide, and adopting enzymolysis treatment to extract cornel polysaccharide, the method has the advantages of moderate conditions, easy removal of impurities, furthest maintained activity of polysaccharide, and the like.
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