Interaction Study of Lipopeptide Biosurfactant viscosin with DPPC and Cholesterol by Langmuir Monolayer Technique

Langmuir monolayers at the air/water interface are frequently used to establish a pseudo-physiological environment needed to study peptides and proteins properties to mimic their interaction with the cell membrane. The self-assembled monolayers formed at the air/water interface that respond to external stimuli and re-structure upon interaction with viscosin peptide in controlled environment have been studied in details. The mechanical and thermodynamic properties of the resulting monolayer are also influenced by temperature and pressure of the resulting mixture. Herein, two distinct pathways were found to contribute to equilibrium formation of DPPC and Cholesterol monolayers. Our data provided an estimate for Gibbs free energy of mixing, the elastic modulus and the classification of the final mixing behavior. Fluorescence microscopy (FM) imaging was also used to assess the morphological and structural changes within the fabricated monolayers. A molecular biological view has been established between our in...