Fetal genetic abnormalities noninvasive detection

The present invention relates to a method without invasive detection of fetal genetic abnormalities by sequencing and large-scale sequencing nucleotides from a maternal biological sample. Also provided are methods to remove due to differences in GC content caused by chromosome sequencing results GC bias. The invention not only makes more accurate detection, and for detecting a given chromosome aneuploidy disorder integrated approach e.g. fetal XO, XXX, XXY, XYY, etc. inclusive.