Single Nucleotide Polymorphisms in MRP2 Gene and Their Significance on Chemosensitivity of Advanced Ovarian Cancer

Objective:To analyze the frequent single nucleotide polymorphisms(SNP)in MRP2 gene among 89 Japanese women with advanced ovarian cancer,and to investigate their significance in chemo-sensitivity to platinum-based chemoth erapy.Methods:Eighty-nine patients with advanced ovarian cancer(stage Ⅲ 77 cases,stage IV 12 cases) were treated with platinum-based or platinum-taxane combined chemotherapy 6～8 courses after cyto-debulking surgery.Sixty-two patients were complete regression(CR)or partial regression(PR),while 27 were no change(NC)or progressed disease(PD).To investigate the clinical significance of SNP in MRP2 gene,three of MRP2 variant alleles promoter(C-24T,exon 10(G1240A),and exton 28(C3972T) were analyzed by using one-base extension method.Results:The SNPs of MRP2 in promoter(C-24T)and exon 28(C3972T)were not associated with response to chemotherapy(CR+PR vs NC+PD),while variant in exon 10(G1249A) was significantly correlated with response to chemotherapy.The mutant alleles of MRP2 in exon 10 was significantly related to sensitiveness of ovarian cancer to therapeutic agents(P0 05).Conclusions:Our results indicate that SNPs of MRP 2 gene in exton 10 may affect their functions and thus the efficacy of chemotherapy against advanced ovarian cancer in Japanese women.