Decarboxyl Enzyme by High Performance Liquid Chromatography

To establish a HPLC method for the determination of teracycliny residual in α-aceryl lactic decarboxyl enzyme. Samples were extracted by EDTA-Mcllvaine buffer and cleaned up by Oasis HLB column.The column was washed by methanol-ethyl acetate.The elution was dried under nitrogen, dissolved in mobile phase. The mycotoxins were separated on a ACR(4.6mm×150mm×5μm) and using mobile phase of oxalic acid-acetonitrile-methanol, the flow rate was 1ml per minute. The limits of detection(LOD) of tetracycline was 0.04043mg/kg, the results showed that the sample was in good linear at the range of 0.0904-15.07mg/kg(r=0.9998). The average recovery rate was77.5%, and the RSD was 2.6%. The method is simple, sensitive, accurate and good reproducibility, proved to be applicable in determination of tetracycline residual in food.