Induction of ICAM-1 and HLA-DR expression by IFN-gamma in malignant melanoma cell lines.

Two human malignant melanoma cell lines, Malme-3M and SK-Mel-28, were analyzed for their ability to induce the expression of intercelluar adhesion molecule 1 (ICAM-1) and human leukocyte antigen (HLA)-DR molecules on their cell surfaces as well as at the transcriptional level before and after treatment with interferon Both cell lines demonstrated a high percentage (>99%) of ICAM-1 expression regardless of treatment. Before treatment, Malme-3M cells barely expressed HLA-DR molecules (50%) of HLA-DR expression. Both cell lines displayed elevated levels of HLA-DR expression in a time dependent manner after treatment. However, these two cell lines have been shown to respond differentially to The molecular mechanism underlying such a differential behavior was investigated, and HLA-DR gene regulation was studied at the transcriptional level. Treatment with led to the steady-state mRNA augmentation of the HLR-DR gene. The HLA-DRA mRNA augmentation was similar in both cell lines, whereas in Malme-3M, did not augment the rate of transcription of the HLA-DRB gene as much as in SK-Mel-28. Data from this study established the fact that the melanoma cell lines displayed a differential susceptibility to on the modulation of HLA-DR molecules, and this modulation was transcriptionally regulated.