ASSA13-03-24 Isolation of Rat Coronary Arterial Smooth Muscle Cells and Preliminary Study of the Characteristics of the Voltage Dependent Potassium Channel

Objective To establish a stable and efficient enzyme separation method to isolation of SD rat coronary artery smooth muscle cells (CASMCs), and to study on the voltage dependent potassium ion channels (K V ). Methods CASMCs were isolated by a “three-step” acute enzyme separation method and identified by immunofluorescence. KV currents were recorded by the patch-clamp technique. Results (1) An abundance of CAMSCs with good reactivity were obtained by this method. (2) At the test potential of +60 mV, the KV current average current densities were 34.35 ± 2.53 pA/pF (n = 5). After added 4-AP, the KV current average current densities were down to 3.55 ± 0.47 pA/pF at the same test potential ( P Conclusions This method can acquire a large number of CASMCs which can be used on the study of ion channels.