Capture library for detecting 50 tumor-relevant hotspot mutation genes and kit

The invention discloses a kit and a method for detecting 50 tumor-relevant hotspot mutation genes. According to the method, a capture method of 207 hotspot mutation regions of 50 relevant genes (including oncogenes and cancer suppressor genes) and a preparation method of a liquid-phase hybridization library used in capturing are researched aiming at oncology. The method particularly comprises the steps of selecting sequence information of 207 hotspot mutation regions, designing a PCR primer, preparing a biotin labeled liquid-phase hybridization library by virtue of a PCR method, hybridizing the biotin labeled liquid-phase hybridization library with a constructed target genome DNA library, and carrying out high-throughput sequencing and bioinformatics analysis on captured fragments, so as to obtain the mutation condition of nucleic acid sequence sites of a sample. By virtue of the method provided by the invention, high-quality sequencing data can be obtained, the relatively high capturing efficiency and the relatively good uniform capturing of the target sites can be realized, the disadvantages of low efficiency, poor uniformity and the like of solid phase hybridization are solved, and the sequencing cost is greatly lowered.