Cytotoxicity evaluation of orthodontic miniscrews

The aim of this study was to determine the cytotoxicity induced by orthodontic miniscrews as received from manufacturer, after exposure to fluorides, and after exposure to the oral environment. Eighty-four miniscrews from Brazilian manufacturers (SIN, INP, and NEODENT) were divided into seven groups (n=12): G1 – SIN as received from manufacturer; G2 – SIN immersed for fifteen days in NaF 0.05%; G3 – SIN exposed to oral environment; G4 – INP as received from manufacturer; G5 – INP immersed for fifteen days in NaF 0.05%; G6 – Neodent as received from manufacturer; G7 – Neodent immersed for fifteen days in NaF 0.05%. Their cytotoxicity was evaluated by direct and indirect exposure tests using the wild S. cerevisiae. Oxidative stress was also evaluated through colony color assay. The results showed non-significant reduction in cell viability on direct or indirect exposure to miniscrews.