Progress of Simple Signal Readout-based Point-of-Care Testing

Abstract As a kind of convenient, fast, and online analysis technology, point-of-care testing (POCT) technology has been widely used in disease diagnosis, health management, environmental monitoring and emergency response analysis. To date, it is a hot spot to develop novel strategy for the detection of various targets based on the simple, rapid and sensitive signal readout and different signal production, transfer and amplification strategies in the field of POCT. The introduction of some nanozymes and functionalized nanomaterials with special catalytic properties further accelerate the application of simple signal readout such as color, temperature, pressure, weight and distance, which greatly promotes the development of simple readout-based POCT methods, and expands the analytical applications of such methods. In this paper, we summarized the principles, classifications, and the applications of these emerging POCT methods for the analysis of life-related targets and reviewed their current challenges and future application prospects.