P157 Monitoring the distribution and genotypic diversity of Burkholderiales bacteria in Russian cystic fibrosis patients in the year of the COVID-19 pandemic

B. cenocepacia ST709 and A. ruhlandii ST36 and 261 are epidemic and the most dangerous for Russian CF patients. Strict infection control helped to preclude further spread of epidemic strains. However, during the 2020 SARS-CoV-2 pandemic it was vital to monitor Burkholderiales in the lung microbiome of CF patients. The samples (sputum/aspirate/BAL/culture) of 66 patients (28 children, 38 adult) of 9 months - 34 years old were collected and analysed by MLST protocols. Burkholderiales were identified in the samples of 23 patients (26.3% of children and 46.4% of adults). Among adult patients, Achromobacter (A. xylosoxidans, A. ruhlandii, A. pulmonis) was the most abundant in the lung microbiome in 7 cases. In the other 4 patients, the A. xylosoxidans was an additional bacterium with the prevalence of P. aeruginosa. The lung microbiome of the youngest patient from this group was the most diverse and included Streptococcus sp., A. xylosoxidans, P. aeruginosa, M. abscessus. Another adult patient had a co-infection with Achromobacter (genogroup 3) and B. multivorans (ST891). A co-infection (A. xylosoxidans ST127/B. cenocepacia ST1772) was revealed in CF infant on the first year of life, too. Timely therapy helped to normalise the lung microbiome. Only one child was infected with epidemic A. ruhlandii ST261 dominated in the microbiome. Other children had A. ruhlandii ST263 or A. xylosoxidans as additional bacteria against the background of Prevotella sp., Granulicatella sp. or E. coli. B. cenocepacia was detected in the samples of 6 children. Epidemic ST709 was diagnosed in one child. Children from the Far East and the Volga Federal District demonstrated ST which are endemic and epidemic for these regions (ST241 and 208, respectively). Burkholderiales are detected in the samples of both children and adult CF patients. The lung microbiome monitoring is important for timely identification and eradication of Burkholderiales to prevent the chronisation of the lung infection.