Generation process and morphological changes of arterioles after implantation of porous silk fibroin films into back skin of rats.

Objective:To explore the regenerate process and the morphological changes of the arterioles after the implantation of the porous silk fibroin film (PSFF),and to afford basic theoretical and experimental basis for the rational design of the PSFF wound-repairing biomaterials.Method:Sixteen adult male rats were selected and divided into 8 groups randomly.The porous silk material were harvested at 5,7,10,13,15,18,23 and 25 days,after separately implanted into the back skin of each rat.The growing state of the arterioles within and around the porous silk material was detected by means of tissue slices,and the changes of the arteriolar density were analysised.Result:There were the arteriole angiogenesis in the adjacent region between the material and the tissue at 7 d after the implantation.The arteriolar density in the adjacent region between the material and the tissue,18d after the implantation,was close to the arteriolar one,10 d after the implantation,and the arteriolar density in porous silk fibroin film continued to an increasing tendency.The arterioles were distributed more evenly at 25 d after the implantation,and the density was consistent with the one in the porous silk fibroin film.Conclusion:There were the arteriolar angiogenesis around and gradually into the porous silk material during the early period after operation.And there were the arteriolar intussusceptive angiogenesis supposed to remodel the network of the vessels during the mid-and late period after operation.