Research on Polymorphism Relationship of Th17/Treg Cell and Its Factors with STAT4 Gene in AITD Patients with Different Iodine Nutritional Status

Background: Research has shown that STAT gene SNP is related to the pathogenesis of T1D, SLE, RA and AITD. Objective: To investigate the correlation of the SNP of Th17/Treg cell and its cytokines with STAT4 in the PBMC of AITD patients with different iodine nutritious status. Method: 100 GD and HT patients who are selected from Cangzhou Central Hospital and Cangzhou People’s Hospital from September 2019 to August 2020, respectively, 60 healthy cases, adopt flow cytometer and quantitative real-time PCR to test the Th17and Treg cell ratio and proportion in PBMC and the expression level of ROR-γt and 3 (Foxp3) mRNA, and use ELISA method to test IL-17 and TGF-β level in the serum. Utilize SASP-PCR technology to test SNP locus (rs7574865) of STAT4 gene, and calculate the genotype and allele frequency. Result: There is some correlation between Th17/Treg cell & its factors in PBMC in the AITD patients and STAT4 rs7574865; compared with healthy control group, the difference between STAT4 rs7574865 G/T &T/T genotype and G&T allele frequency is of statistic significance (χ2 is 6.128 and 5.613 respectively, and the average value of P is <0.05), and may increase the occurrence risk of AITD, but has no correlation with iodine nutritious status. Conclusion: AITD patients not only have Th17 and Treg cell imbalance and the corresponding factor changes but also correlate to STAT4 rs7574865 susceptibility, and the cellular immunity and gene SNP may participate in the disease occurrence process of AIDT together.